Biotium/CF Dye & Biotin SE Protein Labeling Kits/92209/3-labelings

来自 : 发布时间：2024-05-13

哪些发射过滤器适合与 GelRed® 和 GelGreen® 一起使用？常见问题解答Medium & CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色其他 DNA /RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)提出问题使用 GelRed® 的溴化乙锭过滤器； GelGreen® 使用 SYBR® Green 或黄色过滤器。或者，长通黄色滤光片可与 GelRed® 和 GelGreen® 一起使用。请查看 GelRed® 和 GelGreen® 的发射光谱以获得更具体的波长。查看更多常见问题解答哪些仪器可用于检测 GelRed® 和 GelGreen®？常见问题解答 分类细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂& CoverGrip™ 盖玻片密封胶外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架站insOther DNA/ RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)Ask a questionGelRed® 与标准紫外透照仪（302 或 312 nm）兼容。GelGreen® 在 250-300 nm 之间具有足够的吸收，在 500 nm 左右有强吸收峰。 GelGreen® 兼容 254 nm 紫外透照仪或带可见光激发的凝胶读取器，例如 Dark Reader 或 488 nm 激光凝胶扫描仪。查看更多常见问题解答我应该在我的 6X DNA 上样缓冲液中添加多少 GelRed® 或 GelGreen®？常见问题解答MixesEverBrite™ 封片剂和 CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器&细胞骨架染色剂其他 DNA/RNA 凝胶染色剂 PMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducerTyramide Signal Amplification (CF® Dye & Other Tyramides)提出问题我们不建议将 GelRed® 或 GelGreen® 直接添加到上样缓冲液中，因为这会导致条带迁移不准确。 Biotium 提供专为该应用设计的 6X GelRed® Prestain 上样缓冲液，但我们不推荐将它们用于需要精确确定 DNA 条带大小的应用。为了最准确地确定 DNA 条带大小，我们建议使用 GelRed® 后染色（有关详细信息，请参阅 GelRed® 协议）。查看更多常见问题解答GelRed®/GelGreen® 染色后溶液是否可以重复使用？常见问题解答& CoverGrip™ 盖玻片密封胶外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架站insOther DNA/ RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kits二抗TrueBlack® Background ReducersTyramide S信号放大 (CF® Dye & Other Tyramides)问一个问题是的。但是，如果灵敏度降低，请使用新的染料溶液。查看更多常见问题解答染色后是否需要去染步骤？常见问题解答类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂和 CoverGrip ™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他DNA/RNA 凝胶StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)问一个问题没有，但如果背景高，可以用水脱色。查看更多常见问题解答GelRed®/GelGreen® 可以用于 Southern 或 Northern 印迹吗？常见问题解答Medium & CoverGrip™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色其他 DNA /RNA Gel StainsPMA and PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)提出问题GelRed® 已经过验证可用于 Southern 印迹。我们建议使用后染色方案进行印迹应用。查看更多常见问题解答This kit contains everything you need to label antibodies or other proteins with a fluorescent CF™ dye succinimidyl ester (CF dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.

This kit contains everything you need to label antibodies or other proteins with a fluorescent CF™ dye succinimidyl ester (CF dye SE) or biotin succinimidyl ester (biotin SE). Each kit contains reagents for three labeling reactions of up to 1 mg protein per reaction.

It’s available with the following CF™ dyes: Biotin, CF™350, CF™405S, CF™405M, CF™405L, CF™488A, CF™532, CF™543, CF™555, CF™568, CF™594, CF™633, CF™640R, CF™647, CF™660C, CF™660R, CF™680, CF™680R, CF™750, and CF™770.

CF DYE & BIOTIN SE PROTEIN LABELING KITS: Product & SKU Summary

ProductConjugationEx/EmSizeSKUPurchase

Biotin CF Dye & Biotin SE Protein Labeling Kits	Biotin	N/A	3 labelings	92224	Purchase 92224
CF350 CF Dye & Biotin SE Protein Labeling Kits	CF350	347/448 nm	3 labelings	92210	Purchase 92210
CF405L CF Dye & Biotin SE Protein Labeling Kits	CF405L	395/545 nm	3 labelings	92228	Purchase 92228
CF405M CF Dye & Biotin SE Protein Labeling Kits	CF405M	408/452 nm	3 labelings	92212	Purchase 92212
CF405S CF Dye & Biotin SE Protein Labeling Kits	CF405S	404/431 nm	3 labelings	92211	Purchase 92211
CF488A CF Dye & Biotin SE Protein Labeling Kits	CF488A	490/515 nm	3 labelings	92213	Purchase 92213
CF532 CF Dye & Biotin SE Protein Labeling Kits	CF532	527/558 nm	3 labelings	92208	Purchase 92208
CF543 CF Dye & Biotin SE Protein Labeling Kits	CF543	541/560 nm	3 labelings	92209	Purchase 92209
CF555 CF Dye & Biotin SE Protein Labeling Kits	CF555	555/565 nm	3 labelings	92214	Purchase 92214
CF568 CF Dye & Biotin SE Protein Labeling Kits	CF568	562/583 nm	3 labelings	92215	Purchase 92215
CF594 CF Dye & Biotin SE Protein Labeling Kits	CF594	593/614 nm	3 labelings	92216	Purchase 92216
CF633 CF Dye & Biotin SE Protein Labeling Kits	CF633	630/650 nm	3 labelings	92217	Purchase 92217
CF640R CF Dye & Biotin SE Protein Labeling Kits	CF640R	642/662 nm	3 labelings	92225	Purchase 92225
CF647 CF Dye & Biotin SE Protein Labeling Kits	CF647	650/665 nm	3 labelings	92218	Purchase 92218
CF660C CF Dye & Biotin SE Protein Labeling Kits	CF660C	667/685 nm	3 labelings	92219	Purchase 92219
CF660R CF Dye & Biotin SE Protein Labeling Kits	CF660R	663/682 nm	3 labelings	92223	Purchase 92223
CF680 CF Dye & Biotin SE Protein Labeling Kits	CF680	681/698 nm	3 labelings	92220	Purchase 92220
CF680R CF Dye & Biotin SE Protein Labeling Kits	CF680R	680/701 nm	3 labelings	92226	Purchase 92226
CF750 CF Dye & Biotin SE Protein Labeling Kits	CF750	755/777 nm	3 labelings	92221	Purchase 92221
CF770 CF Dye & Biotin SE Protein Labeling Kits	CF770	770/797 nm	3 labelings	92222	Purchase 92222

References

Download a list of CF dye references.