EverBrite™ 封片剂、湿式和硬式封片剂是否与 Alexa Fluor® 染料或 Cy® 染料兼容？常见问题解答StainEvaGreen® 染料和预混液EverBrite™ 封片剂和 CoverGrip™ 盖玻片密封胶外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶
染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶检测微生物学：细菌和酵母染料 Mix-n-Stain™
抗体标记试剂盒NucView ® 胱天蛋白酶-3 酶底物细胞器和细胞骨架染色剂其他 DNA/RNA 凝胶染色剂用于活性 PCR 的 PMA 和 PMAxx™产品运输、储存、保质期和溶解度蛋白质检测和分析刃天青细胞活性测定RNAstorm™ 和 DNAstorm™ FFPE 提取试剂盒二级 AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification（CF® 染料和其他酪胺）提出问题是的，EverBrite™ 封片剂与多种染料兼容。与 VectaShield® 不同，EverBrite™ 不会干扰 Alexa Fluor® 647、Cy®3 或 Cy®5 等花青基荧光团。AlexaFluor 是 Thermo Fisher Scientific 的注册商标； Cy Dye 是 Cytiva 的注册商标； VectaShield 是 Vector Laboratories 的注册商标。查看更多常见问题解答EverBrite™ 封片剂的折射率是多少？常见问题解答™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他DNA/RNA 凝胶StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide信号放大（CF® 染料和其他酪胺）提出问题EverBrite™ 封片剂 (23001) 和 EverBrite™ 封片剂与 DAPI (23002) 的折射率为 1.46。 EverBrite™ Hardset Mounting Medium (23003) 和 EverBrite™ Hardset Mounting Medium with DAPI (23004) 的初始折射率均为 1.38，固化 24 小时后增至 1.42，4 天后增至 1.46，之后保持恒定查看更多常见问题LAMP 应使用什么浓度的 EvaGreen？常见问题解答 类别
细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA
定量试剂盒DNAzure® 可见 DNA 凝胶染色剂 EvaGreen® 染料和预混液EverBrite™ 封片剂和 CoverGrip™盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/ RNA 凝胶染料 PMA和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Am增生（CF® 染料和其他酪胺）问一个问题 仪器读取荧光信号时，0.5X 至 1X EvaGreen® 通常用于 LAMP 应用。对于肉眼检测，可以使用增加浓度的 EvaGreen®。例如，Lee, S. H. 等人。测试了高达 100X EvaGreen®，发现 10X EvaGreen® 最适合他们的检测。Lee, S. H. 等人。正面。微生物学，2017 年 1 月 9 日 |https://doi.org/10.3389/fmicb.2016.02166 查看更多常见问题解答EvaGreen® 染料溶液的浓度是多少？常见问题解答 类别细胞凋亡、坏死和细胞活力试剂盒CellBrite® 和 MemBrite® 膜和细胞表面染色剂CF® 染料 DNA 和 RNA 定量试剂盒DNAzure® 可见 DNA 凝胶染色剂EvaGreen® 染料和预混液EverBrite™ 封片剂和 CoverGrip™盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他 DNA/ RNA 凝胶染料 PMA和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal 扩增（CF® 染料和其他酪胺）提出问题 EvaGreen® 染料以 20X (25 uM) 的水（目录号 31000）或 2000X（2.5 mM）的 DMSO（目录号 31019）提供。其他配方可供产品被许可人使用，请通过 btinfo@
Biotium.com 咨询。另请参阅我们用于 qPCR 的即用型 EvaGreen® 预混液。查看更多常见问题解答Fast Probe Master Mix 中的 ROX 浓度是多少？常见问题解答™ 盖玻片密封剂外泌体和 EV 染色GelRed® 和 GelGreen® 核酸凝胶染色剂GelRed® 和 GelGreen® 故障排除离子指示剂和螯合剂荧光素酶测定微生物学：细菌和酵母染料 Mix-n-Stain™ 抗体标记试剂盒NucView® Caspase-3 酶底物细胞器和细胞骨架染色剂其他DNA/RNA 凝胶StainsPMA 和 PMAxx™ for viability PCRProduct shipping, storage, shelf life, & solubilityProtein Detection & AnalysisResazurin Cell Viability AssayRNAstorm™ and DNAstorm™ FFPE extraction kitsSecondary AntibodiesTrueBlack® Background ReducersTyramide Signal Amplification (CF® Dye & Other Tyramides)提问 ROX 浓度为 35 nM，适用于低 ROX 仪器。如果您使用的是高 ROX 仪器，您可以在 TE
缓冲液（目录号 29052）中添加 25 mM ROX，该缓冲液可单独购买（目录号 29052）。查看更多常见问题解答Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
Live-or-Dye™ Fixable Viability Staining Kits are designed for discrimination between live and dead cells during flow cytometry. Live/dead stains are useful probes to include when analyzing cell surface protein expression by flow cytometry, because they allow intracellular fluorescence signal from dead cells with permeable plasma membranes to be excluded from analysis.
Live-or-Dye™ Fixable Viability Stains are cell membrane impermeable amine-reactive dyes. The dyes are able to enter into dead cells that have compromised membrane integrity and covalently label free amines on intracellular proteins. On live cells, the dyes react with surface proteins, but these are much less abundant than intracellular proteins, resulting in low staining levels compared to dead cells. The dye labeling is extremely stable, allowing the cells to be fixed and permeabilized without loss of fluorescence or dye transfer between cells. The Live-or-Dye™ staining protocol has been optimized to maximize live/dead discrimination with minimal live cell staining, in order to prevent interference with immunostaining.
Biotium offers a selection of eight different Live-or-Dye™ viability stains spanning the fluorescence spectrum, for maximal flexibility in multi-color analysis.
Catalog No.Product DescriptionLaser lineEmission filterAbs/Em
| 32002, 32002-T | Live-or-Dye™ 350/448 | 355 nm | DAPI or Violet | 347/448 nm |
| 32003, 32003-T | Live-or-Dye™ 405/452 | 405 nm | Pacific Blue | 408/452 nm |
| 32009, 32009-T | Live-or-Dye™ 405/545 | 405 nm | AmCyan | 395/545 nm |
| 32004, 32004-T | Live-or-Dye™ 488/515 | 488 nm | FITC | 490/515 nm |
| 32005, 32005-T | Live-or-Dye™ 568/583 | 488 or 561 nm | PE | 562/583 nm |
| 32006, 32006-T | Live-or-Dye™ 594/614 | 488 or 561 nm | PE-Texas Red | 561/624 nm |
| 32007, 32007-T | Live-or-Dye™ 640/662 | 633 or 640 nm | APC | 642/662 nm |
| 32008, 32008-T | Live-or-Dye™ 755/777 | 633 or 640 nm | APC-Cy7 | 755/777 nm |
| 32010, 32010-T | Live-or-Dye NucFix™ Red | 488 or 532 nm | PE-Texas Red | 520/610 nm |
References
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