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Biotium/HotStart Polymerase Modification Kit/29054-T/trial-size
Thisisaspecialorderproduct.PleaseinquireforavailABIlity.
TheHotStartPolymeraseModificationKitprovidesaneasywaytoreversiblymodifylysineresiduesofthermostableDNApolymerase,renderingtheenzymeinactive.Themodificationisreversedafterheatingto>90°C.HotStartmodificationofDNApolymeraseforPCRpreventsamplificationofnon-specificPCRproductsduetolowstringencyannealingofprimersatlowtemperatureduringreactionassembly.
Thetrialsizekit(29054-T)issufficientformodifying0.1mgpolymerase,whiletheregularsizekit(29054)issufficientformodifying0.5mgpolymerase.
BiotiumalsoofferstheWarmStart™EnzymeModificationKit(catalogno.29053)fortemperaturecontrolofnon-thermostableenzymes.Forconcentrationmeasurementofpolymerasebeforemodification,weoffertheAccuOrangeProteinQuantitationKitforhighlysensitivefluorometricmeasurementofpurifiedproteinconcentration.
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