2XForget-Me-Not™UniversalProbeMasterMixisahigh-performanceproductforfluorescentprobe-basedPCRapplications,includingquantitationandSNPgenotyping.Thiskitissuitableforallfluorescentprobe-basedtechnologies,includinghydrolysisprobes(suchasTaqMan®anddual-labeledBHQ®probes)anddisplacementprobes(likeMolecularBeacons).Forget-Me-Not™UniversalProbeMasterMixshowsexcellentconcordanceofresultsinsingleplexandmultiplexreactions,hasbroadinstrumentcompatibility,andcanbeusedinbothstandardandfastprotocols.AseparatekitisalsoavailablewithROX,ifyourinstrumentrequiresROXreferencedye.
Cheetah™HotStartTaqDNAPolymeraseisBiotium’sproprietarychemicallymodifiedhot-startDNAPolymerase.Cheetah™Taqisfullyactivatedin2minuteswithhighactivityrecovery,makingitparticularlysuitableforfastPCR.Cheetah™Taqiscompletelyinactiveatroomtemperature.
Forget-Me-Not™TrackingBuffer.The40XForget-Me-Not™TrackingBuffercontainsaninertbluedye.YouhavethechoiceofaddingTackingBuffertothemastermix,totheDNAtemplate,ornottousethetrackingbufferinyourreactions.AdditionofTrackingBuffertoyourmastermixallowsyoutoeasilydistinguishwellscontainingreactionmixfromemptywells.Ontheotherhand,addingTrackingBuffertoyourDNAtemplatesamplesallowsyoutotrackwhichreactionshavehadtemplateaddedwhileyousetupyourPCRreactions.
SNPgenotypingusingdual-labeledBHQ1Plus®hydrolysisprobes.DNAsamplesweregenotypedinreactionscontaining450nMofeachprimerand100nMofeachprobe(Allele1probe-FAM/BHQ®-1,Allele2probe-CALFluor®Orange560/BHQ®-1)onaQuantStudio®5system(ThermofisherScientific).
SingleplexandtriplexPCRofGAPDH,ACTBandTFCRfromhumangenomicDNA.PCRdetectionofGAPDH,ACTBorTFCRinsingleplexreactions,ortogetherintriplexreactions.Reactionscontained20ngofhumangenomicDNA,400nMofeachprimer,and200nMofeachhydrolysisprobe(GAPDH-FAM/BHQ®-1,ACTB-CALFluor®Orange560/BHQ®-1,TFCR-CALFluorRed610/BHQ®-2.
TaqMan®isa registeredtrademarkofRocheDiagnostics.BHQ®isaregisteredtrademarkofBiosearchTechnologies.
References
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