Highperformancemastermixwith2-colorreactioncomponenttrackingsystem,atagreatprice

• HighperformanceqPCRmastermixatafractionofthecostofsimilarproducts,aslowas$20/mL
• 2-colorsystemhelpsyoucatchpipettingmistakes,savingtime,reagents,DNAsamples,and$$$
• EvaGreen®Dye–bettersignalthanSYBR®Green,saferandmoreenvironmentallyfriendly
• Cheetah™HotstartTaq– fullyactivatedaftertwominutesat95°Cforfastcyclingprotocols
• SeparateROXforoptimizingwithanyPCRinstrument

Forget-Me-Not™qPCRMasterMixisahot-startEvaGreen®dye-basedmastermixforuseinrealtimePCRapplicationsandDNAmeltcurveanalysis.Forget-Me-Not™featuresauniquecombinationofamastermixcontainingalowconcentrationofbluedye,plusaDNAtemplatebuffercontainingahigherconcentrationofbluedye.Whenyouaddthe2XForget-Me-Not™MasterMixtoyourreaction,itappearslightblue(Figure1,left).Then,whenyouaddtemplatecontainingForget-Me-Not™TemplateBuffertothereaction,thecolorturnsdarkblue(Figure1,right).Forget-Me-Not™allowsyoutoseeataglancewhetheryouforgottoaddmastermixortemplatetoanyofyourreactions,soyoucancatchpipettingmistakesandavoidwastingtime,reagents,andyourpreciousDNAsamples.The2XForget-Me-Not™MasterMixalsocanbeusedwithouttheTemplateBufferifyouprefer.

KitComponents:

Forget-Me-Not™EvaGreen®qPCRMasterMix

The2XMasterMixcontainsEvaGreen®dye,Cheetah™HotStartTaqDNAPolymerase,dNTPs,andalowconcentrationofaninertbluedye,whichallowstheusertodistinguishwellscontainingreactionmixfromemptywells.YoucanusethemastermixwithorwithouttheForget-Me-Not™TemplateBuffer.

Forget-Me-Not™TemplateBuffer

The40XTemplateBuffercontainsahighconcentrationofinertbluedye.AddingthetemplatebuffertoyourDNAtemplatesamplesallowsyoutoeasilytrackwhichreactionshavehadtemplateaddedwhenyouraresettingupyourPCRreactions.

Note:Forget-Me-Not™qPCRMasterMix(31041)doesnotincludeROXreferencedye.ForinstrumentsthatrequireeitheraloworhighconcentrationofROXreferencedye,weofferForget-Me-Not™qPCRMasterMixwithROX(31042),whichincludesaseparatevialofROXReferencedye,andinstructionsforhowmuchROXtoadddependingonwhichprotocolandinstrumentyouareusing.DownloadtheForget-Me-Not™qPCRMasterMixProtocolfordetails.

Features:

EvaGreen®dye

AuniqueDNA-bindingdyewithfeaturesidealforbothqPCRandHighResolutionMelting®(HRM)analysis*.EvaGreen®dyebindstodsDNAviaanovel“release-on-demand”mechanism,whichpermitstheuseofarelativelyhighdyeconcentrationinqPCRwithoutPCRinhibition(Ref.1).

AuniquefeatureofEvaGreen®dyeisitssafety.DNA-bindingdyesareinherentlydangerousduetotheirpotentialtocausemutation.Withthisinmind,Biotium’sscientistsdesignedEvaGreen®dyesuchthatitcannotcrosscellmembranes,thuspreventingthedyefrombeingincontactwithgenomicDNAinlivecells.AllothercommercialPCRdyesenterintocellsinamatterofminutes.SYBR®GreenI,forexample,hasbeenshowntobeenvironmentallymoretoxicthanethidiumbromide,awell-knownmutagen(Ref.2).IndependentlabshaveconfirmedthatEvaGreendyeisnonmutagenic,noncytotoxicandsafetoaquaticlifefordirectdisposalinthedrain.Fordetails,downloadtheEvaGreen®DyeSafetyReport.

AnaddedbenefitofanEvaGreen®-basedmastermixisthatyoucananalyzeyourPCRproductbygelelectrophoresiswithouttheneedtoaddanotherDNA-bindingdyetoeitheryourloADIngbufferorgel.TheEvaGreen®dyeinthemastermixcanactasaDNApre-stain,permittingdirectvisualizationofDNAbandsfollowingelectrophoresis.

Cheetah™HotStartTaq

Cheetah™HotStartTaqDNAPolymeraseisBiotium’sproprietarychemically-modifiedhot-startDNAPolymerase.Cheetah™Taqiscompletelyinactiveatroomtemperature,andisfullyactivatedafter2minutesat95°C,makingitparticularlysuitableforfastcyclingPCRprotocols.

Peaceofmind,plusPCRperformance

Forget-Me-Not™qPCRMasterMixhasbeenformulatedforfastcyclingPCRparameters,butcanalsobeusedwithregularcyclingprotocols.Forget-Me-Not™qPCRMasterMixperformsaswellasourFastEvaGreen®MasterMix,andaswellasorbetterthanQiagen’sQuantiNova®SYBR®GreenPCRMasterMixinareal-timePCRassay(Figure2),whilemakingiteasyforyoutomakesureyourreactionsaresetupcorrectlythefirsttime,everytime.

AlsoseeBiotium’sotherqPCR reagentsandkits.

EvaGreen®DyeandCheetah™HotStartTaqDNAPolymerasearecoveredunderUSandinternationalpatents.QuantiNova®isaregisteredtrademarkofQiagenGroup.SYBR®GreenisaregisteredtrademarkofMolecularprobes,Inc./LifeTechnologies.*HRM®isaregisteredtrademarkofIdahoTechnologies,Inc./BioFireDefense,LLCanditsusemayrequirealicense.