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当前位置: 首页 > 产品中心 > Fluorescent_dyes > Biotium//31015-1/5-x-1-ml
商品详细Biotium//31015-1/5-x-1-ml
Biotium//31015-1/5-x-1-ml
Biotium//31015-1/5-x-1-ml
商品编号: 31015-1
品牌: Biotium Inc
市场价: ¥6640.00
美元价: 3984.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium’s PCR master mix delivers superior performance even with the most challenging samples tested.

Fast Plus EvaGreen® qPCR master mix is a ready-to-use hot-start mix for nucleic acid quantitation and melt curve analysis of PCR amplicons. Formulated using our environmentally safe EvaGreen® dye and fast-activating chemically-modified hotstart enzyme, Cheetah™ Taq, Biotium’s PCR master mix delivers superior performance even with the most challenging samples tested. For detailed information, click the download tab to view the protocol and product flyer pdf files. The master mix is offered in three formats containing different amounts of ROX reference dye for use with different PCR instruments (without ROX, low ROX, and high ROX). View the product protocol to find the master mix that is recommended for your instrument.

EvaGreen dye and applications are covered under patent US patent nos. 7,803,943 and 7,776,567 and pending international patents. Cheetah Taq is covered by pending US and international patents.

References

Mao, et al. Characterization of EvaGreen Dye and the implication of its physicochemical properties for qPCR applications. BMC Biotechnology 7, 76 (2007).

Download a list of Selected EvaGreen References.

品牌介绍
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。