FastEvagreen®qPCRMasterMixisaPCRreagentsuitableforqPCRusingafastcyclingprotocol.ThereagentsystemusestwoofourbreakthroughPCRtechnologies,Evagreen®qPCRdyeandCheetah™TaqhotstartDNApolymerase,todeliversuperiorresultswhencomparedtoothercommercialmastermixes.

• Evagreen®dye:Evagreen®DNA-bindingdyeselectivelybindstodsDNAviaanovel“release-on-demand”mechanismthatensureslowPCRinhibitionandthuspermitsthedyetobeusedatarelativelyhighconcentrationformeltcurveanalysissignal.BecausethedyeisspectrallysimilartoFAMorSYBR®GreenI,thedyeiscompatIBLewithallcommercialqPCRinstruments.Moreover,unlikeSYBR®GreenIandSYBRGreenER®,Evagreen®dyeisextremelystable.Finally,Evagreen®dyeisnonmutagenicandnoncytotoxic.

• Cheetah™Taq:Cheetah™Taqisanew,chemicallymodifiedhotstartDNApolymerase.UnlikeAmplitaqGold®,Cheetah™Taqcanbeactivatedinaslittleastwominutesunderstandardhotstartcondition(i.e.,94°CinpH8-9Tris)withhighactivityrecovery.Alsounlikeantibody-basedhotstartpolymerases,suchasPlatinum®Taq,Cheetah™TaqiscompletelyinactiveatroomtemperatureandhasintrinsicallylowDNAcontamination.Inaddition,Cheetah™Taqisgenerallymorestableat-20°Cor4°CthanAmpliTaqGold®,ensuringthatthemastermixmaintainsitspeakperformancefollowingstorageatlowtemperature.

FastEvagreen®qPCRMasterMixisa2Xmastermix,andissuppliedwithaseparatevialofROXreferencedye,forusewithhighROX,lowROX,ornoROXinstruments.Seetheproductinformationsheetformoreinformation.

EvaGreendyeandapplicationsarecoveredunderUSpatentnos.7,803,943and7,776,567andpendinginternationalpatents.CheetahTaqiscoveredbypendingUSandinternationalpatents.