Formalin-fixedtissuesamplesareachallengeforRNAextraction,oftenresultinginlowyieldsofamplifiableRNAandpoorperformanceinsubsequentstepsinvolvingenzymaticmanipulation,includingreversetranscriptionandsequencinglibrarypreparation.
PoweredbyproprietaryCAT5™technology,theRNAstorm™extractionkitenhancestheremovalofformaldehyde-induceddamageandprovidesRNAwithhigheryieldandquality,betterintegrity,andgreateramplifiABIlity.
WhetheryouareperformingRNA-seq,qPCR,microarrayorothergeneexpressionanalysis,theRNAstorm™kitisyourbestchanceforsuccess.
WealsooffertheDNAstorm™kitforextractionofhigh-qualityDNAfromFFPEtissuesamples.
AdvantagesoftheRNAstorm™Kit
HigherYieldsofAmplifiableRNA
SignificantimprovementsinRNAyieldandquality(asmeasuredbyamountofamplifiableRNA)areseenusingaprototypeoftheRNAstorm™kitonFFPEsamplesfromvarioustissuesandranginginagefrom1976to2015.
ComparisonofRNArecoverybyquantitativeRT-PCRfromFFPEtissues.“Q”representscompetitivecommercialFFPEextractionkits.
HigherIntegrityRNA
IncreasedRNAintegrityisobservedforRNAextractedusingtheRNAstorm™kit.
Anexemplarycomparisonwithapopularcommercialkitisshownbelow(AgilentBioanalyzerRNA6000nano)
IncreasesinDV200(thepercentageofRNAwithlength>200nt)wereseenforavarietyoftissuesamples.
