Biotin-16-dUTPcanbeenzymaticallyincorporatedintoDNAvianicktranslation,randompriming,or3′endterminallabeling.Theterminaldeoxynucleotidyltransferase(TdT)-mediatedbiotin-dUTPnickend-labeling(TUNEL)methodhasbeencommonlyusedforapoptosisstudies.Thenumber’16’isthenumberofatomsinthelinkerbetweenbiotinanddUTP.Biotiumalsooffersbiotin-11-dUTP(catalogno.40029)andbiotin-20-dUTP(catalogno.40030).Thelengthofthelinkeraffectstheincorporationefficiencyofthebiotin-dUTPprobeintoDNAusingDNApolymerases,anditalsoaffectsbiotin/avidinorbiotin/streptavidin.Ingeneral,theshorterthelinker,themoreefficientlythebiotin-dUTPisincorporatedintoDNAbyDNApolymerases.Ontheotherhand,alongerlinkershouldfacilitateinteractionbetweenbiotinandavidinorstreptavidin.
- 1mMsolutioninpH7.5Tris-HClbuffer
- Storeat-20°C
- C32H48N7O18P3Sli4
- MW:971.5
Biotin-16-dUTPisalsoavailableasalyophilizedsolid(catalogno.40022-1).
References
1.AnalBiochem193,2(1991).
2.JCellBiol119,493(1992).
3.NeuroReport,7,61(1995).
4.Biotechniques19,800(1995).
5.JournalofVirologicalMethods(2013),http://dx.doi.org/doi:10.1016/j.jviromet.2013.12.019
6.TheOpenGenomicsJournal5,18-29(2012)
