The AccuGreen™ High Sensitivity dsDNA Quantitation Solution is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ assay is specific for dsDNA, and can quantify DNA samples in the range of .01-10 ng/uL. For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear™ or AccuBlue™ NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range.
The AccuGreen™ High Sensitivity dsDNA Quantitation Solution includes AccuGreen™ dye and assay buffer. Biotium also offers the AccuGreen™ High Sensitivity dsDNA Quantitation Kit (catalog no. 31066), which includes dye, buffer, and a set of 0 and 10 ng/uL calf thymus dsDNA standards, for use in the Qubit® pre-programmed dsDNA High Sensitivity assay.
Features
- Optimized for use with the Qubit® Fluorometer
- Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
- Linear range in assay: 0.1-100 ng dsDNA
- Green fluorescence (Ex/Em 502/523 nm)
Components
- AccuGreen™ Dye, 200X
- AccuGreen™ Buffer, 1X
Kit sizes
- The 500 assay kit (31068) contains enough reagents to perform 1,250 reactions, including standards (allowing the measurement of approximately 500 DNA samples)
- The 100 assay trial kit (31068-T) contains enough reagents to perform 250 reactions, including standards (allowing the measurement of approximately 100 DNA samples)
Other DNA Quantitation Kits:
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Features |
|---|---|---|---|
| AccuBlue™ NextGen | 1 pg*-3 ng | 468/507 | Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity. |
| AccuClear™ Ultra High Sensitivity | 30 pg-250 ng | 468/507 | This kit offers great sensitivity and the widest linear range. |
| AccuBlue™ High Sensitivity | 0.2-100 ng | 485/530 | Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye. |
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher. |
| AccuBlue™ Broad Range | 2-2000 ng | 350/460 | For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity. |
References
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