生物素/31066-T/100分析
商品编号：
31066-T
品牌：
Biotium Inc
市场价：
¥1500.00
美元价：
900.00
产品分类：
荧光染料
公司分类：
Fluorescent_dyes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL.
The AccuGreen™ High Sensitivity dsDNA Quantitation Kit is designed for use with handheld fluorometers such as the Qubit® fluorometer from Thermo Fisher. The AccuGreen™ kit is specific for dsDNA, and can quantify DNA samples in the range of 0.01-10 ng/uL. For high-sensitivity DNA quantitation using a fluorescent plate reader, we recommend using our AccuClear™ or AccuBlue™ NextGen dsDNA Quantitation Kits, which provide unprecedented sensitivity, accuracy and wide linear range.
Features
- Optimized for use with the Qubit® Fluorometer
- Uses the pre-programmed Qubit® dsDNA High Sensitivity assay setting
- Linear range in assay: 0.1-100 ng dsDNA
- Green fluorescence (Ex/Em 502/523 nm)
Kit components
- AccuGreen™ Dye, 200X
- AccuGreen™ Buffer, 1X
- AccuGreen™ Standard 1 (0 ng/uL)
- AccuGreen™ Standard 2 (10 ng/uL)
Kit sizes
- The 500 assay kit (31066) contains enough reagents to perform 1,250 reactions, including standards (allowing the measurement of approximately 500 DNA samples)
- The 100 assay trial kit (31066-T) contains enough reagents to perform 250 reactions, including standards (allowing the measurement of approximately 100 DNA samples)
Other DNA Quantitation Kits:
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Features |
|---|---|---|---|
| AccuBlue™ NextGen | 1 pg*-3 ng | 468/507 | Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity. |
| AccuClear™ Ultra High Sensitivity | 30 pg-250 ng | 468/507 | This kit offers great sensitivity and the widest linear range. |
| AccuBlue™ High Sensitivity | 0.2-100 ng | 485/530 | Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye. |
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher. |
| AccuBlue™ Broad Range | 2-2000 ng | 350/460 | For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity. |
References
n/a
品牌介绍
★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。
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