Biotium/AccuBlue宽量程双链DNA定量试剂盒，带DNA标准/31007/1500分析
商品编号：
31007
品牌：
Biotium Inc
市场价：
¥4720.00
美元价：
2832.00
产品分类：
荧光染料
公司分类：
Fluorescent_dyes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
TheAccuBlue™BroadRangedsDNAQuantitationKitofferssensitiveandselectivedetectionofpurifieddsDNAsampleswithminimaleffectsfromcommoncontaminants. Thekitprovidesalineardetectionrangefrom2–2000ngand iscompatIBLewithfluorescencemicroplatereaders.
TheAccuBlue™BroadRangedsDNAQuantitationKitofferssensitiveandselectivedetectionofpurifieddsDNAsampleswithminimaleffectsfromcommoncontaminants. Thekitprovidesalineardetectionrangefrom2–2000ngand iscompatiblewithfluorescencemicroplatereaders.
Features
- Broadlineardetectionrange,from2-2000ng
- Rangecanbeextendedto4000ngwithminorlossoflinearity
- λEx/λEm:350/460nm
- TheAccuBlue™BroadRangeassayissuperiortotheQuant-iT®BroadRangedsDNAandotherdetectionkitsdueto similarsensitivitybutwithmoreconvenienceandimproveddynamicrange
Kitsizes
- The1500assaysizekitincludesAccuBlue™BroadRangeQuantitationSolutioncomponentsandasetof9pre-dilutedcalfthymusdsDNAstandards. withsufficientreagentstoperform1500assaysbasedona96-wellmicroplateformat.
- ThetrialsizekitincludesoneDNAstandardsolutionwithinstructionsforpreparingasetofstandardsbyserialdilution.AccuBlue™BroadRangeQuantitationSolutionisalsoavailableseparately(cat.no.31009)forcustomerswhowishtousetheir ownDNAstandards.
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingthequbit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
AccuBlue™technologyiscoveredbygrantedU.S.andinternationalpatents.
References
1.PLoSONE(2013),doi:10.1371/journal.pone.0080613
2.JThrombThrombolytic(2014),doi:10.1007/s11239-014-1137-7
3.PLoSONE(2015),doi:10.1371/journal.pone.0117792
4.MolecularEcology(2015),doi:10.1111/mec.13167
5.PLoSONE(2015),doi:10.1371/journal.pone.0132137
品牌介绍
★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。
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