TheAccuBlue™NextGendsDNAQuantitationKitprovidesanunprecedentedlevelofsensitivityindsDNAdetection.TheAccuBlue™NextGen dsDNAQuantitationassayisthemostsensitiveandaccurateDNAquantitationmethodforultralowamountsofDNA. Withadetectionlimitaslowas1pgDNAina96-wellplatereaderassay(seeTable1),AccuBlue™NextGenoutperformsrivalssuchasPicoGreen®insensitivityandaccuracy.SpeciallydesignedforscientistswhoneedtoquantifyextremelydiluteorpreciousDNA,AccuBlue™NextGenisidealforuseinpreparingsamplesforapplicationssuchasNextGenerationSequencing(NGS)anddigitalPCR.
TheAccuBlue™NextGen dsDNAquantitationassayisdesignedforusewithfluorescence96-wellplatereadersequippedwithexcitationandemissionfiltersfordetectinggreenfluorescence.TheuniquespectralpropertiesofAccuBlue™NextGenDyemakeitespeciallywell-suitedforusewithinstrumentswithblueLEDexcitationsources. BecausetheAccuBlue™NextGenassayissosensitive,resultswillvarydependingonmanyfactors,suchasmicroplatereader(seeTable1),assayvolume,andpipetaccuracy.
TheAccuBlue™NextGendsDNAQuantitationKit containsAccuBlue™NextGenDye,Enhancer,assaybuffer,anddsDNAstandard,withinstructionsforpreparingasetofstandardsbyserialdilution.
Kitcomponents
- AccuBlue™NextGenDye,400X
- AccuBlue™NextGenEnhancer,100X
- AccuBlue™NextGenBuffer,1X
- AccuBlue™NextGenDNAStandard,300pg/uL
Features
- Linearrange:1-3000pgdsDNA
- Greenfluorescence(Ex/Em460/507nm)
- Unrivaledsensitivity
- AssaystABIlity:preparedworkingsolutioncanbestoredforupto6monthsinthefridgeforaddedconvenience.
- GreenfluorescentdyeisaperfectmatchforblueLEDexcitationsources
- GreenfluorescencedetectioncompatIBLewithfluorescencemicroplatereaders,handheldfluorometers,and Nanodrop®fluorospectrophotometers.
| reader | assayformat(assayvolume) | lowestDNAdetected |
|---|---|---|
| BMGCLARIOStar | 96-well(200uL) | 1pg |
| BMGCLARIOStar | 384-well(50uL) | 0.6pg |
| BiotekSynergyH1m | 96-well(200uL) | 5pg |
| SpectraMaxGeminiXS | 96-well(200uL) | 5pg |
| SpectraMaxi3 | 384-well(50uL) | 1.25pg |
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingthequbit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
AccuBlueNextGentechnologyiscoveredbyU.S.patents.
References
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