The AccuBlue™ High Sensitivity dsDNA Quantitation Kit offers sensitive and selective detection of purified dsDNA samples with minimal effects from common contaminants. The assay provides a with sufficient reagents to perform 1000 assays based on a 96-well microplate format. AccuBlue™ High Sensitivity Quantitation Solution also is available with pre-diluted DNA standards (Catalog #31006).
The assay is compatible with microplate readers, spectrofluorometers and hand-held minifluorometers. Note that while the assay is spectrally compatible with Qubit® fluorometers from Thermo Fisher, it may not be compatible with the high sensitivity dsDNA program on all Qubit® models (see our DNA Quantitation FAQs for more information). For the Qubit® we recommend our AccuGreen™ High Sensitivity Kit (cat. no. 31066), which was specially formulated for that instrument.
Features
- linear detection range from 0.2 – 100 ng
- λEx/λEm 485/530 nm
- Safer than PicoGreen® or Quant-iT® dsDNA detection kits: the high sensitivity reagent is impermeable to cell membranes
Other DNA Quantitation Kits:
| dsDNA Quantitation Kit | Linear detection range (dsDNA in assay) | Ex/Em (nm) | Features |
|---|---|---|---|
| AccuBlue™ NextGen | 1 pg*-3 ng | 468/507 | Quantitation of extremely precious or dilute samples. Optimal for sensitive applications such as NGS or digital PCR. *Limit of detection in the range of 1 pg to 5 pg depending on instrument sensitivity. |
| AccuClear™ Ultra High Sensitivity | 30 pg-250 ng | 468/507 | This kit offers great sensitivity and the widest linear range. |
| AccuBlue™ High Sensitivity | 0.2-100 ng | 485/530 | Quantitation of dsDNA from 0.2-100 ng, with a safer, membrane impermeable DNA dye. |
| AccuGreen™ High Sensitivity | 0.1-100 ng | 502/523 | Quantitation of DNA using the Qubit® fluorometer from Thermo Fisher. |
| AccuBlue™ Broad Range | 2-2000 ng | 350/460 | For quantitation of high concentration DNA in a broad linear range with blue fluorescence. Assay can be extended to 4000 ng with minor loss of linearity. |
AccuBlue™ technology is covered by granted U.S. and international patents.
References
1. BMC Oral Health (2014), doi: 10.1186/1472-6831-14-157
