TheAccuBlue™ HighSensitivitydsDNAQuantitationKitofferssensitiveandselectivedetectionofpurifieddsDNAsampleswithminimaleffectsfromcommoncontaminants.Theassayprovidesa withsufficientreagentstoperform1000assaysbasedona96-wellmicroplateformat.AccuBlue™HighSensitivityQuantitationSolutionalsoisavailablewithpre-dilutedDNAstandards(Catalog#31006).
Theassay iscompatIBLewithmicroplatereaders,spectrofluorometersandhand-heldminifluorometers.Notethatwhiletheassayisspectrallycompatiblewithqubit®fluorometersfromThermoFisher,itmaynotbecompatiblewiththehighsensitivitydsDNAprogramonallQubit®models(seeourDNAQuantitationFAQsformoreinformation).FortheQubit®werecommendourAccuGreen™HighSensitivityKit(cat.no.31066),whichwasspeciallyformulatedforthatinstrument.
Features
- lineardetectionrangefrom0.2–100ng
- λEx/λEm485/530nm
- Saferthan PicoGreen®orQuant-iT®dsDNAdetectionkits:thehighsensitivityreagentisimpermeabletocellmembranes
OtherDNAQuantitationKits:
| dsDNAQuantitationKit | Lineardetectionrange(dsDNAinassay) | Ex/Em(nm) | Features |
|---|---|---|---|
| AccuBlue™NextGen | 1pg*-3ng | 468/507 | Quantitationofextremelypreciousordilutesamples.OptimalforsensitiveapplicationssuchasNGSordigitalPCR.*Limitofdetectionintherangeof1pgto5pgdependingoninstrumentsensitivity. |
| AccuClear™UltraHighSensitivity | 30pg-250ng | 468/507 | Thiskitoffersgreatsensitivityandthewidestlinearrange. |
| AccuBlue™HighSensitivity | 0.2-100ng | 485/530 | QuantitationofdsDNAfrom0.2-100ng,withasafer,membraneimpermeableDNAdye. |
| AccuGreen™HighSensitivity | 0.1-100ng | 502/523 | QuantitationofDNAusingtheQubit®fluorometerfromThermoFisher. |
| AccuBlue™BroadRange | 2-2000ng | 350/460 | ForquantitationofhighconcentrationDNAinabroadlinearrangewithbluefluorescence.Assaycanbeextendedto4000ngwithminorlossoflinearity. |
AccuBlue™technologyiscoveredbygrantedU.S.andinternationalpatents.
References
1.BMCOralHealth(2014),doi:10.1186/1472-6831-14-157
