TheYeastViabilityStainingKitisafluorescentassaytodetermineyeastviability.Eachkitcontainstwofluorescentcomponents,CFDye-labeledConcanavalinA(ConA)andLive-or-Dye™viabilitydye.Thereare3differentcolorcombinationstochoosefrom(ifthereisadifferentcolorcombinationthatyouwouldprefer,emailusattechsupport.Biotium.com).
Live-or-Dye™viabilitydyesarecellmembraneimpermeableamine-reactivedyesdesignedfordiscriminationbetweenliveanddeadcellsduringflowcytometryormicroscopy.Theyareabletoenterintodeadcellsthathavecompromisedmembraneintegrityandcovalentlylabelfreeaminesonintracellularproteins.Thisdyelabelingisextremelystable,allowingthecellstobefixedandpermeabilizedwithoutlossoffluorescenceordyetransferbetweencells.
ConcanavalinA(ConA)isawidelyusedlectinthatselectivelybindstoa-mannopyranosylanda-glucopyranosyresidues,foundinthecellwallofyeastandfungi.Itislabeledwithoneofoursuperiorbright,photostableCF™dyes.
Afterstainingyeastwiththiskit,bothlivinganddeadcellswillhavecellwallsstainedwithConA,whileonlydeadcellswillalsohavethecytoplasmstainedwiththeLive-or-Dye™.
KitComponents(for1000assays):
- Live-or-Dye™(solid),4vials
- ConA(solid),5vials
- anhydrousDMSO,250uL
References
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