PMA-Lite™LEDPhotolysisDeviceisspecificallydesignedforphotoactivationofpropidiummonoazide(PMA)andothersimilardyes.ItcanalsofunctionasageneralphotolysisdevicetoprovidecontinuousIlluminationto1.5-2mL-sizedvialsinacontrolledmanner.Thedevicecanholdupto18vials.MultipleLEDlightsarepositionedtoprovideevenandmaximalilluminationfromboththesidesandbottomtoallvials.Aninternalfanisusedtoreducetheheatgeneratedfromthelightstoensurenearroomtemperaturephotolysis.Thedevicehasatimerthatcanbesetfor10,15,20or30minutesofcontinuousphotolysis.Theunithas120/240Vinternalconverter.AuniversaloutletadapterisincludedforcustomersoutsideofNorthAmerica.ThePMA-Lite™LEDPhotolysisDeviceiscoveredbyawarrantyfortwoyearsfromdateofpurchase.Seepage2oftheProductionInformationSheetforwarrantydetailsandinstructionsforobtainingservice.
PMA(catalognos.40019and40013)isaphoto-reactivedyethatbindstodsDNAwithhighaffinityandcanbeusedfortheselectivedetectionofviablepathogensbyqPCR. PMAxx™(catalogno.40069)isanewandimprovedphotoreactivedyeforviABIlityPCR.LearnmoreaboutPMAandPMAxx™.
WealsooffertheGlo-Plate™Blue,aflatLEDilluminatorforviabilityPCR. Whileourstandard PMA-Lite™ LEDphotolysisdevicewasdesignedforusewithmicrocentrifugetubes,theGlo-Plate™BluedeviceallowsformoreflexIBLeassayset-ups,includingmulti-wellplatesforhigh-throughputassays.ThebaseoftheGlo-Plate™BluechambercontainsblueLEDlightstoppedwithadiffuser,allowingforevenilluminationacrosssamples.
ReceiveafreetrialsizeofPMAxx™ withpurchaseofthePMA-Lite™LEDPhotolysisDevice.
PMA-Lite™LEDPhotolysisDevice.Eachdevicecomeswithoneremovablebluetubeadapter/rack.
OtherPMA-relatedproducts:
ForviabilityPCRofgram-negativebacteriawehighlyrecommendusingournewPMAEnhancer(31038)inconjunctionwithPMA.PMAEnhancerforGramNegativeBacteriawasdesignedtoimprovePMA-mediateddiscriminationbetweenliveanddeadgram-negativebacteria.Whenasequencefromagram-negativebacteriaisamplifiedbyPCR,samplespre-treatedwithEnhancershowadecreaseinthesignalfromdeadcells,withnochangeinthesignalfromlivecells.
Biotiumalsoprovidesstrain-specificPMAReal-TimePCRBacterialViabilitykitswithvalidatedprimersfor5selectedpathogens:M.tuberculosis,S.aureus,MRSA,Salmonella,andE.coliO157:H7.Thesekitsprovideeverythingthatyouneedfortheselectivedetectionofyourfavoritespeciesoflivebacteriabyreal-timePCR.Don’tseeyourfavoritestrain?Letusknowattechsupport@biotium.com.
Real-TimePCRBacterialViabilityKits
References
1.Nocker,A.,Cheung,C.Y.,andKamper,A.K.(2006).Comparisonofpropidiummonoazidewithethidiummonoazidefordifferentiationoflivevs.deadbacteriabyselectiveremovalofDNAfromdeadcells.J.MicrobioMeth.67(2),310-320.
2.Front.Microbiol.(2015),doi:10.3389/fmicb.2015.00295
3. ApplMicrobiolBiotechnol(2015),doi:10.1007/s00253-015-6715-x
DownloadthefullPMAReferenceList.
