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主营:主营:荧光指示剂,生化产品,荧光计,Fluo-3, Fura-2, BCECF,JC-1等产品
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当前位置: 首页 > 产品中心 > Fluorescent_dyes > Biotium/Bacterial Viability and Gram Stain Kit/32001/200-assays
商品详细Biotium/Bacterial Viability and Gram Stain Kit/32001/200-assays
Biotium/Bacterial Viability and Gram Stain Kit/32001/200-assays
Biotium/Bacterial Viability and Gram Stain Kit/32001/200-assays
商品编号: 32001
品牌: Biotium Inc
市场价: ¥4320.00
美元价: 2592.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
TheBacterialViABIlityandGramStainKitcontainsthreecomponents:CF™488A-WGA,DAPI,andEthD-IIIsolutionsfordistinguishingbetweengram-negativeandgram-positive,aswellasliveversusdeadbacteria.

TheBacterialViabilityandGramStainKitcontainsthreecomponents:CF™488A-WGA,DAPI,andEthD-IIIsolutionsfordistinguishingbetweengram-negativeandgram-positive,aswellasliveversusdeadbacteria.CF™488A-labeledwheatgermagglutininstainstheN-acetylglucosamineofthepeptidoglycanlayerofgram-positivebacteria1withgreenfluorescence(Ex/Em490/515nm).Thebacterialviabilitycomponentoftheassayisbasedontheintegrityofthecellmembrane.EthD-IIIisanucleicacidbindingdyethatismembraneimpermeable,whichselectivelystainscellswithcompromisedplasmamembraneswithredfluorescence(Ex/Em530/620nm).AllcellsstainbluewiththemembranepermeableDNAdyeDAPI(Ex/Em358/461nm).Onekitissufficient200assayswhenusedaccordingtothekitprotocol.

TheBacterialViabilityandGramStainKithasbeentestedonthefollowingbacterialspecies:Bacillussubtilissubsp.subtilis,Escherichiacoli,Micrococcusluteus,Pseudomonasfluorescens,andStaphylococcusepidermidis.

References

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品牌介绍
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。