Biotium/NucView 530 Caspase-3底物，1 mM，PBS/10408/100微升-免疫在线蚂蚁淘旗下平台

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商品详细Biotium/NucView 530 Caspase-3底物，1 mM，PBS/10408/100微升

Biotium/NucView 530 Caspase-3底物，1 mM，PBS/10408/100微升

商品编号： 10408

品牌： Biotium Inc

市场价： ¥6420.00

美元价： 3852.00

产地：美国(厂家直采)

公司：

产品分类：荧光染料

公司分类： Fluorescent_dyes

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

NucView™530Caspase-3Substrate,1mMinPBS,providesaconvenienttoolfordetectingapoptosisinintactcellsbasedoncaspase-3/7activityusingeitherconfocalmicroscopyorflowcytometry.

Incontrasttootherfluorogeniccaspasesubstratesorfluorescentcaspaseinhibitorbased(FLICA)assays,NucView™caspase-3substratescanbeusedtodetectcaspase-3/7activitywithinindividualintactcellswithoutinhibitingapoptosisprogression.NucView™substratesconsistofafluorogenicDNAdyecoupledtothecaspase-3/7DEVDrecognitionsequence.Thesubstrate,whichisinitiallynon-fluorescent,penetratestheplasmamembraneandentersthecytoplasm.Inapoptoticcells,caspase-3/7cleavesthesubstrate,releasingthehigh-affinityDNAdye,whichmigratestothecellnucleusandstainsDNAwithfluorescence.Thus,NucView™Caspase-3Substratesarebifunctional,allowingdetectionofcaspase-3/7activityandvisualizationofmorphologicalchangesinthenucleusduringapoptosis.Thestainingisalsoformaldehyde-fixable.

NucView™530Caspase-3Substratestainsapoptoticcellnucleiwithorangefluorescence(Ex/Em528/563nm,Figure1),fordetectionintheCy®3channelbyfluorescencemicroscopy(Figure2)orthePEchannelbyflowcytometry(Figure3).NucView™530canbeusedformulti-colorimagingwithblue,green,orfar-redfluorescentprobes.Notethatwhenexcitedbythe488nmlaserline,NucView™530alsofluorescesintheFITCchannel,andthereforecannotbeanalyzedtogetherwithgreenprobesbyflowcytometry.

NucView™530Caspase-3Substrateisavailableasa1mMsolutioninDMSO(catalogno.10406)orPBS(phosphate-bufferedsaline).ThesubstrateinPBSisformulatedforuseincellsthataresensitivetoDMSOtoxicity.Innon-DMSOsensitivecelltypes,addingDMSOduringthesubstrateincubationmayenhanceNucView™530staining.

Biotiumalsooffersbluefluorogenic NucView™405Caspase-3Substrate,andgreenfluorogenicNucView™488Caspase-3Substrateandkits.

Excitation and emission spectra of NucView 530 dye with dsDNA. — Figure1.ExcitationandemissionspectraofNucView™530dyewithdsDNA.

MitoView Blue, NucView 530, CF488A Annexin MCF7 composite — Figure2.Detectionofmitochondrialmembranepotential,caspaseactivity,andapoptosisinMCF-7cells.Cellswereeitheruntreated(A)ortreatedwithstaurosporineovernighttoinduceapoptosis(B),thenstainedwithMitoView™Blue,NucView™530Caspase-3Substrate,andCF™488AAnnexinVfor30minutesat37^oCincellculturemediumwithnowash.HealthycellsshowmitochondrialstainingwithMitoViewBlue(cyan).ApoptoticcellsloseMitoView™Bluestaining(cyan),andshowNucView™520stainingofnuclei(red)andCF™488AAnnexinVstainingofcellmembranes(green).

NucView 530, CF405M Annexin V staurosporine dose curve — Figure3.JurkatcellsweretreatedwithDMSO(negativecontrol,blue)or0.1uM,0.25uM,or1uMstaurosporine(red)toinduceapoptosisinthepresenceofNucView™530andCF™405MAnnexinVforfourhoursat37^oCbeforeanalysisonaBDLSRIIflowcytometer.StainingwithNucView™530andCF™405MAnnexinVincreasewithincreasingconcentrationofstaurosporine.

CydyeisaregisteredtrademarkofGEHealthcare.

品牌介绍

★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。

公司介绍

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蛋白质检测酵母污渍其他酶底物胞质示踪剂 DNA结合染料细胞膜染料核酸凝胶污渍及相关产品活力测定受体/离子通道毒素和探针

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