>
产品中心 >
Fluorescent_dyes >
Biotium/NucView 488 Caspase-3 Enzyme Substrate, 1 mM in PBS/10403-T/10-ul
NucView™488Caspase-3Substrateisanovelcellmembrane-permeablefluorogeniccaspasesubstratedesignedfordetectingcaspase-3/7activitywithinlivecellsinrealtime. Visit ourProductVideopage toseeavideoofNucView™488inaction.
Unlikeconventionalcaspaseassays,NucView™488Caspase-3Substratedetectscaspase-3/7activitywithinindividualintactcellswithoutinhibitingcaspaseactivity.ThesubstrateconsistsofafluorogenicDNAdyeandaDEVDsubstratemoietyspecificforcaspase-3/7.Thesubstrate,whichisbothnon-fluorescentandnonfunctionalasaDNAdye,rapidlycrossescellmembranestoenterthecytoplasm,whereitiscleavedbycaspase-3toformahigh-affinityDNAdyethatstainsthenucleusbrightgreen.Thus,theNucView™488Caspase-3Substrateisbi-functional,allowingdetectionofintracellularcaspase-3/7activityandvisualizationofchangesinnuclearmorphologyduringapoptosis.Thefluorescentstainingproducedinresponsetocaspase-3/7activityiscompatIBLewithsubsequentfixationandpermeABIlizationforimmunostaining.
NucView™ 488Caspase-3Substrate,isofferedasa1mMsolutioninDMSO(catalogno.10402)orPBS(phosphate-bufferedsaline).ThesubstrateinPBSisformulatedforuseincellsthataresensitivetoDMSOtoxicity.Innon-DMSOsensitivecelltypes,addingDMSOduringthesubstrateincubationmayenhanceNucView488staining.Inadditiontostand-alonesubstratestocksolutions,weoffera NucView™488assaykit withdetailedapplicationprotocols.BiotiumalsooffersthebluefluorescentNucView™405Caspase-3Substrateforusewiththe405nmlaserlineandtheorangefluorescentNucView™530forimagingintheCy®3channel.DownloadtheNucView™Flyerformoreinformation.
NucView™enzymesubstratetechnologyiscoveredbyU.S.patents.
References
Cen,etal.DEVD-NucView488:anovelclassofenzymesubstratesforreal-timedetectionofcaspase-3activityinlivecells.TheFASEBJournalpublishedonlineFebruary8,2008.
Downloadafulllistofreferences:ValidatedcelllinesforNucView488withreferences
