Coelenterazineeisasyntheticderivativeofcoelenterazine,andisasuperiorsubstrateforRenillaluciferase. Coelenterazineegeneratesnearly40%morelightthannativecoelenterazinedoeswhiletheinitiallightintensityoftheformerismorethan7timeshigherthanthatofthelatter. Theemissionspectrumofcoelenterazinee/Renillaluciferasehastwopeakscenteredat418and475nm,respectively.
Coelenterazineeisalsoagoodsubstrateforapoaequorinand,comparedwithcoelenterazinenative,thee-aequorincompelxformedfromcoelenterazineehasafasterluminescencerisetimeandshowstwoemissionpeaksat405and465nmrespectively,withtheratioofthepeakheightsdependentoncalciumconcentrationintherangepCa5-7. Thedualemissionmakescoelenterazinee/apoaequorinpossIBLetobeusedforcalciumdetectionusingtheratioofthetwoemissionintensities.
- YellowsolidsolubleinMeOHorEtOH
- Storeat-20°Candprotectfromlight
- C28H23N3O3
- MW:449.50
References
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