Coelenterazine (native form) is a luminescent enzyme substrate for apoaequorin and Renilla luciferase. The aequorin complex has been used as an excellent calcium indicator that can measure a broad concentration range of calcium from ~0.1 uM to >100 uM (References 1-4). Renilla luciferase has been widely used as a reporter protein. Renilla luciferase/coelenterazine has also been used as the bioluminescence donor in bioluminescence resonance energy transfer (BRET) (5) to study protein-protein interactions. Other uses of coelenterazine include chemiluminescent detection of superoxide anion and peroxynitrite in cells or tissues (6-9). Biotium also offers water soluble Aquaphile™ Coelenterazine. See the coelenterazine flyer under downloads for a comparison of coelenterazine analogs.
- Yellow solid soluble in MeOH or EtOH
- Store at -20°C and protect from light
- C26H21N3O3
- MW: 423
- [55779-48-1]
References
For in-vivo imaging:
For reporter assay:
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