Coelenterazine(nativeform)isaluminescentenzymesubstrateforapoaequorinandRenillaluciferase.Theaequorincomplexhasbeenusedasanexcellentcalciumindicatorthatcanmeasureabroadconcentrationrangeofcalciumfrom~0.1uMto>100uM(References1-4).Renillaluciferasehasbeenwidelyusedasareporterprotein.Renillaluciferase/coelenterazinehasalsobeenusedasthebioluminescencedonorinbioluminescenceresonanceenergytransfer(BRET)(5)tostudyprotein-proteininteractions.Otherusesofcoelenterazineincludechemiluminescentdetectionofsuperoxideanionandperoxynitriteincellsortissues(6-9).BiotiumalsoofferswatersolubleAquaphile™Coelenterazine.Seethecoelenterazineflyerunderdownloadsforacomparisonofcoelenterazineanalogs.
- YellowsolidsolubleinMeOHorEtOH
- Storeat-20°Candprotectfromlight
- C26H21N3O3
- MW:423
- [55779-48-1]
References
Forin-vivoimaging:
Forreporterassay:
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