
细菌活细胞和死细胞生物素/活力/细胞毒性检测试剂盒/30027/100-1000-检测
商品编号:
30027
品牌:
Biotium Inc
市场价:
¥5680.00
美元价:
3408.00
产品分类:
荧光染料
公司分类:
Fluorescent_dyes
联系Q Q:
3392242852
电话号码:
4000-520-616
电子邮箱:
info@ebiomall.com
商品介绍
Thiskitprovidesatwo-colorfluorescencestainingonbothlivebacteria(green)anddeadbacteria(red)usingtwoprobesDMAOandEthD-III.
Thiskitprovidesatwo-colorfluorescencestainingonbothlivebacteria(green)anddeadbacteria(red)usingtwoprobesDMAOandEthD-III.DMAOisagreen-fluorescentnucleicaciddyewhichstainsbothliveanddeadbacteria.EthD-IIIisared-fluorescentnucleicaciddyethatselectively stainsdeadbacteriawithdamagedcellmembranes.WithanappropriatemixtureofDMAOandEthD-III,bacteriawithintactcellmembranesshow greenfluorescence,whereasbacteriawithdamagedcellmembranesshowredfluorescence.Thekitissuitableforusewithfluorescencemicroscopesandflowcytometers.Theassayprinciplesaregeneralandapplicabletomostbacteriatypes.For100flowcytometryassaysor1000microscopyassays.
KitComponents:
- 2vials(100uL/vial):DMAO,5mM
- 2vials(150uL/vial):EthidiumHomodimer-III(EthD-III),2mM
Features:
- DualDetection:Detectliveanddeadbacteriacellsinacellpopulationsimultaneously
- Simple&Fast:15mindyeloADIngandmeasurewithoutwashing
- Economic:PerformviABIlityandcytotoxicityassaysatthesametime
- Versatile:AnalysiscompatIBLewithflowcytometersandfluorescencemicroscopesusingpopularsettingsforfluoresceinandpropidiumiodide.
References
n/a
品牌介绍
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。
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