ResazurinCellViabilityAssayoffersasimple,rapid,reliable,sensitive,safeandcost-effectivemeasurementofcellviability.Thiskitperformsatleastaswellasothercommercialresazurin-basedcellproliferationassaykitssuchasalamarBlue®orCellTiter-Blue®.Resazurindetectscellviabilitybyconvertingfromanonfluorescentdyetothehighlyredfluorescentdyeresorufininresponsetochemicalreductionofgrowthmediumresultingfromcellgrowth(1-3).Continuedcellgrowthmaintainsareducedenvironmentwhileinhibitionofgrowthmaintainsanoxidizedenvironment.Reductionrelatedtogrowthcausestheredoxindicatortochangefromoxidized(nonfluorescent,blue)formtoreduced(fluorescent,red)form.Thefluorescentsignalismonitoredusing530-560nmexcitationwavelengthand590nmemissionwavelength.Theabsorbanceismonitoredat570nmand600nm.Thefluorescentorcolorimetricsignalgeneratedfromtheassayisproportionaltothenumberoflivingcellsinthesample.Resazurinsolutionissuppliedready-to-usein25mL(2500assay)or100mL(10,000assay)sizes.
Features:
- Sensitive:Requiringasfewas80cellsforreproducIBLeresults
- Simple:Single-stephomogenousassay
- KineticMonitoring:Reagentsnontoxictocells,thusallowingkineticmonitoring.
alamarBlueisaregisteredtrademarkofTrekBiosystems.CellTiter-BlueisaregisteredtrademarkofPromegaCorporation.
References
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