PathoGreen Histofluorescent Stain is an anionic green fluorescent dye functionally similar to Fluoro-Jade dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons.
PathoGreen Histofluorescent Stain is an anionic green fluorescent dye functionally similar to Fluoro-Jade dyes. These dyes stain degenerating neurons and their processes after exposure to a variety of neurotoxic insults in brain sections and cultured neurons. The mechanism of neuronal staining by anionic fluorescent dyes has not been determined. It has been proposed that the negatively charged dyes bind to positively charged polyamines or other molecules specifically generated in dying neurons.
Degenerating neurons in a section of mouse hippocampus stained with PathoGreen Histofluorescent Stain.
References
1. Neurological Research (2014), doi: 10.1179/1743132813Y.0000000289
品牌介绍
★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。
