TheMTTCellViabilityAssayKitprovidesasimplemethodfordetermininglivecellnumbersusingastandardcolorimetricplatereaders.Determinationoflivecellnumbersisoftenusedtoassesstherateofcellproliferationandcytotoxicitycausedbydrugsandcytotoxicagents.Amongallnon-rADIoactiveviabilityassays,MTTassaydevelopedbyMossmanisoneofthemostversatileandpopularassays.MTTisatetrazoliumsaltthatisturnedintoapurpleformazanproductafterreductionbymitochondrialenzymesthatareonlypresentinmetabolicallyactivelivecells,notindeadcells.Theamountofformazanproductgeneratedisproportionaltothenumberoflivingcellsinthesample.Attheendoftheassay,thecellscontainingtheformazanproductare solubilizedandthenphotometricallyquantifiedat570nm.
AlsoseetheXTTCellViabilityAssayKit,whichdoesnotrequiresolubilizationbeforeabsorbancemeasurement.
References
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