Fireflyluciferaseiswidelyusedasareportergeneforstudyinggeneregulationandfunction,andforpharmaceuticalscreening(1).Itisaverysensitivegeneticreporterduetothelackofanyendogenousluciferaseactivityinmammaliancellsortissuesandtheverynatureofbioluminescence(2).Fireflyluciferaseisa62,000daltonproteinwhichisactiveasamonomeranddoesnotrequiresubsequentprocessingforitsactivity.TheenzymecatalyzesATP-dependentD-luciferinoxidationbyoxygenintooxyluciferinwithemissionoflightcenteredon560nm.Aswithmanyenzymes,fireflyluciferasefollowsMichaelis-Mentenkinetics,andasaresultmaximumlightoutputisnotachieveduntilthesubstratesandco-factorarepresentinlargeexcess.Whenassayedundertheseconditions,lightemittedfromthereactionisdirectlyproportionaltothenumberofluciferaseenzymemolecules.TheFireflyLuciferaseAssayKitisdesignedfordetectionandquantificationoffireflyluciferasereporterenzymefromculturedcellsinasimple,efficientandlinearfashion.
FireflyLuciferaseAssayKit(Lyophilized)performscomparablytoBiotium’sFireflyLuciferaseAssayKit2.0,butincludesassaybufferinlyophilizedformatformoreeconomicalshippingandconvenientstorageat-20°C.
KitComponents:
- D-luciferin
- 5XFireflyluciferaselysisbuffer
- Fireflyluciferaseassaybuffer(lyophilized)
Features:
- SensitivityandLinearity:Linearcorrelationbetweenfireflyluciferaseenzymelevelsandlightoutput
- Convenience:Lyophilizedbufferforeconomicalshippingandconvenientstorage
MoreLuciferaseKits
| Catalognumber | Product | Description |
|---|---|---|
| 30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
| 30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30028 | Steady-Luc™FireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
| 30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
| 99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
| 99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30020 | ATP-Glo™BioluminometricCellViABIlityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
| 22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
References
1.Anal.Biochem.219:169-181(1994).
2.Anal.Biochem.175:5-13(1988).
