4000-520-616
欢迎来到免疫在线!(蚂蚁淘生物旗下平台)  请登录 |  免费注册 |  询价篮
Biotium Inc(授权代理)
主营:主营:荧光指示剂,生化产品,荧光计,Fluo-3, Fura-2, BCECF,JC-1等产品
咨询热线电话
4000-520-616
当前位置: 首页 > 产品中心 > Fluorescent_dyes > Biotium/Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate & Annexin V/30067/50-assays
商品详细Biotium/Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate & Annexin V/30067/50-assays
Biotium/Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate & Annexin V/30067/50-assays
Biotium/Dual Apoptosis Assay with NucView 488 Caspase-3 Substrate & Annexin V/30067/50-assays
商品编号: 30067
品牌: Biotium Inc
市场价: ¥6820.00
美元价: 4092.00
产地: 美国(厂家直采)
公司:
产品分类: 荧光染料
公司分类: Fluorescent_dyes
联系Q Q: 3392242852
电话号码: 4000-520-616
电子邮箱: info@ebiomall.com
商品介绍
This kit provides two apoptosis markers, our novel NucView™ 488 Caspase-3 Substrate and Annexin V conjugated with your choice of red or far-red dye. The kit is used for studying two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

This kit provides two apoptosis markers, our novel NucView™ 488 Caspase-3 Substrate and Annexin V conjugated with a dye, for profiling apoptotic cells and studying the relation of two important apoptosis events, caspase-3 activation and phosphatidylserine (PS) translocation.

Annexin V conjugation for the second marker is available with the following dyes: Sulforhodamine 101 (Texas Red®) Annexin V, CF™594 Annexin V, and CF™640R Annexin V.

The rate of apoptosis varies from cell to cell even within the same population. As a result, various apoptotic events or markers accompanying the apoptotic process also occur differently among cells. Thus, it is important to be able to detect these apoptotic events on an individual cell basis. Traditionally, caspase activity has been detected either using a membrane-impermeable fluorogenic enzyme substrate such as DEVD-R110, or a fluorescently-labeled inhibitor such as a FLICA reagent. In the former case, cell lysis is required, thus precluding the detection of caspase activity in live cells. In addition, such caspase assays measure only the average caspase activity of a highly heterogeneous cell population at a given time. In the latter case, although a FLICA reagent can enter live cells to detect caspase activity, only the initial fluorescent signal following the application of the reagent can truly reflect the enzyme activity or the state of the apoptotic cells because any detected signal after the initial “snapshot” will need to consider the potential interference of the inhibitor to the enzyme and the apoptotic cell itself.

Unlike conventional caspase assays, NucView™ 488 Caspase-3 substrate detects caspase-3 activity within individual whole cells in a non-interfering manner. The substrate consists of a fluorogenic DNA dye and a DEVD substrate moiety specific for caspase-3. The substrate, which is both non-fluorescent and nonfunctional as a DNA dye, rapidly crosses cell membranes to enter the cytoplasm, where it is cleaved by caspase-3 to form a high-affinity DNA dye that stains the nucleus bright green. Thus, the NucView™ 488 caspase-3 substrate is bi-functional, allowing detection of caspase-3 activity and visualization of apoptotic nuclear morphology.

The kit contains reagents sufficient for 50 flow cytometry assays (200 uL assay volume). The number of fluorescence microscopy assays that can be performed with the kit may vary based on the size of culture vessel and staining volume used.

See our full selection of NucView™ substrates and kits.

NucView™ enzyme substrate technology is covered by U.S. patents.

Texas Red® is a registered trademark of Molecular Probes.

References

Cen, et al. DEVD-NucView488: a novel class of enzyme substrates for real-time detection of caspase-3 activity in live cells. The FASEB Journal published online February 8, 2008.
Download a list of NucView references: Validated cell lines for NucView 488 with references

Download a full list of references: Validated cell lines for NucView 488 with references

1. PLoS Genet (2014), doi:10.1371/journal.pgen.1004594

2. Cancer Gene Therapy (2016), doi:10.1038/cgt.2016.18

品牌介绍
★EB(溴化乙锭):溴化乙锭是一种高度灵敏的荧光染色剂,用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号,可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下,SYBR Green I发出微弱的荧光,但一旦与双链DNA结合后,荧光大大增强。因此,SYBR Green I的荧光信号强度与双链DNA的数量相关,可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm,发射波长最大约为520nm。