ThiskitcontainsCF™488AAnnexinVforstainingapoptoticcellsgreen,and7-AADforstainingnecroticcellswithfar-redfluorescence,fordetectionbyflowcytometryorfluorescencemicroscopy.
Phosphatidylserine(PS)istranslocatedfromtheinnertotheoutersurfaceofapoptoticcellsforphagocyticcellrecognition.Thehumananticoagulant,AnnexinV,isa35kDaCa2-dependentphospholipidproteinwithahighaffinityforPS.AnnexinVlabeledwithCF™488A(Abs/Em=490/515nm)canidentifyapoptoticcellsingreenbybindingtoPSexposedontheouterleaflet.OurCF™488Adyeissuperiortofluorescein/FITCasitisnotaffectedbypHandhasmuchbetterphotostABIlity.
7-AADisamembrane-impermeantnucleicaciddyethatisexcludedbylivecells,butstainsnecroticcellsandlateapoptoticcells.7-AAD(Abs/EmwithDNA=543/655nm)emitsinthefar-redspectrumandcanbewellseparatedfromCF™488Aandothergreenemittingdyesusingthe488nmlineofaflowcytometer.
References
- PLOSONE(2016),http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0155450
