CalceinAMisawidelyusedgreenfluorescentcellMarker.CalceinAMismembrane-permeantandthuscanbeintroducedintocellsviaincubation.Onceinsidethecells,calceinAM,anonfluorescentmoleculeitself,ishydrolyzedbyendogenousesteraseintothehighlynegativelychargedgreenfluorescentcalcein.Greenfluorescentcalceinisretainedinthecytoplasminlivecells.CalceinAMhasservedasausefultoolforthestudiesofcellmembraneintegrityandforlong-termcelltracing.TheCalceinAMCellViabilityAssayKitisdesignedtoquantifylivecellnumbersbasedontheirendogenousesteraseactivityandplasmamembraneintegrity.Itisatrueend-pointassayforcellviability.Thefluorescentsignalismonitoredusing485nmexcitationwavelengthand530nmemissionwavelength.Thefluorescentsignalgeneratedfromtheassayisproportionaltothenumberoflivingcellsinthesample.
WealsosupplyCalceinAMinavarietyofpackagingsizesandformats.
KitComponents:
- CalceinAMinanhydrousDMSO
Features:
- Trueend-pointviabilityassay:Detectonlylivecellswithintactplasmamembraneintegrity
- Simple&Fast:30-minassay
References
1.JNeurosciMethods.50,205(1993).
2.JImmunolMethods163,181(1993).
3.CancerLett.87,199(1994).
4.JImmunolMethods178,41(1995).
