Thelossofmitochondrialmembranepotentialisahallmarkforapoptosis.Itisanearlyeventprecedingphosphatidylserineexternalizationandcoincidingwithcaspaseactivation(1,2).JC-1MitochondrialMembranePotentialDetectionKitisusedtomeasuremitochondrialmembranepotentialchangesincells.Innon-apoptoticcells,JC-1accumulatesasaggregatesinthemitochondrialmembranes,resultinginredfluorescence(590nm).Thebrightnessofredfluorescenceisproportionaltothepotentialandvariesamongdifferentcelltypes.However,inapoptoticandnecroticcells,whichhavediminishedmitochondrialmembranepotential,JC-1existsinthegreenfluorescent(529nm)monomericform(3-5).Thiskitprovidesastep-by-stepprotocolandready-to-usereagentsforperformingassaysforflowcytometry,fluorescencemicroscopy,orfluorometricmicroplatereader.
WealsoofferJC-1iodidesaltandJC-1chloridesalt.
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