ViaFluor™LiveCellMicrotubuleStainsarecell-permeabletaxolprobesforimagingthemicrotubulecytoskeletoninlivecells. Theyaresimple,rapidandsensitivestainsthatcanbeimagedwithoutawashstep.ViaFluor™488MicrotubuleStainhasgreenfluorescence(Ex/Em500/515nm)forimagingintheFITCchannel.ViaFluor™647MicrotubuleStainhasfar-redfluorescence(Ex/Em~650/~675nm) forimagingintheCy®5channel,andiscompatIBLewithsuper-resolutionimagingbySIMorSTED.
TaxolbindstopolymerizedtubulinandstABIlizesmicrotubules,resultingininhibitionofinhibitionofmitosis.However,fluorescenttaxolcompoundslikeViaFluor™647arelessdisruptiveofmicrotubuledynamicsandcelldivision,presumablyduetolowerbindingaffinityofthefluorescentprobecomparedtotaxolitself.ViaFluor™647doesnotshowcytotoxicitywithupto24hoursofexposureinimmortalizedcelltypes. ViaFluor™LiveCellMicrotubuleStains cannotbefixedafterstaining,andcannotbeusedwithfixedcellsortissues.
ViaFluor™MicrotubuleStainsaresuppliedasa1000XstocksolutionsinDMSO.Theycomewithavialof100mMverapamil,aneffluxpumpinhibitorthatmayimproveproberetentionandstainingincertaincelltypes.Notethatthisisahigherconcentrationthanpreviouslysuppliedwiththestains,toallowfortheuseofhigherverapamilconcentrations(10-100uM).Theoptimalconcentrationofverapamilmayvarybetweencelltypes.
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[/one_half_last]Cy5isaregisteredtrademarkofGEHealthcare.
