Biotium’sCellBrite™NIRCytoplasmicMembraneDyesareready-to-usedyedeliverysolutionsthatcanbeaddeddirectlytonormalculturemediatouniformlylabelcellsinsuspensionoradherentcultures.

Carbocyaninedyeslabelcytoplasmicmembranesandintracellularmembranestructuresefficientlyandpermanently(1).Theyhavebeenusedastracersincell–cellfusion(2,3),cellularadhesion(4,5),andmigration(6)applicationsduetotheirpropertiesoflowcytotoxicityandhighresistancetointercellulartransfer.However,thelipophilicnatureofthesedyesposeanobstacletouniformcellularlabeling.AlthoughstructurallyrelatedPKHdyeshavebeendevelopedandoptimizedforcelllabeling,theprocedurerequiresmultiplestepsandsubjectscellstoaniso-osmoticmannitolloADIngmedium(7,8). Avarietyofcarbocyaninedyeswithdifferentopticalpropertiesareavailable,allowingcellpopulationstobemarkedwithdistinctfluorescentcolorsforidentificationaftermixing.Doublelabelingcanidentifycellsthathavefusedorformedstableclusters.

CellBrite™NIRCytoplasmicMembraneDyesarenovelnear-infraredcarbocyaninedyesforlabelingthecytoplasmicmembranesoflivingcells.Duetotheirlongemissionwavelengths,near-infraredcellmembranestainscanbeusedtolabelcellsfornear-infraredsmallanimalimagingstudiesfornon-invasiveimagingofcellmigrationandcellhoming(9).Thedyeshavelong18-carbonhydrophobictailsandanadditionalwater-solublegroup.Theseuniquechemicalstructureelementsmakethedyeseasytodissolvewhileprovidinghighlystablecytoplasmicmembranestaining,unliketraditionalcarbocyaninedyeslikeDiI,DiO,andDiR,whichareoftendifficulttodissolveorpronetoprecipitationduringcellstaining.

CellBrite™NIRCytoplasmicMembraneDyesareavailableinthefollowingcolors:NIR680,NIR750,NIR770,andNIR790.

BiotiumoffersotherCellBrite™CytoplasmicMembraneStainsinavarietyofcolors.Formoreinformation,downloadtheproductinformationsheet,orseeFrequentlyAskedQuestionsaboutCellBrite™CytoplasmicMembraneStains.

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