BAPTAanditsderivativesarecalciumchelatorsthatarecommonlyusedtoformcalciumbufferswithwell-definedcalciumconcentrations.ByinjectingthechelatorsintocellsorbyincubatingcellswiththeAMesterformofthechelators,onecancontrolthecytosoliccalciumconcentration,animportantmeanstostudytherolesofcalcium.
KeyadvantagesofthesecalciumchelatorsincluderelativeinsensitivitytowardintracellularpHchangeandfastreleaseofcalcium.BiotiumoffersseveralBAPTAchelatorswithcalciumdissociationconstantscoveringtheBIOLOGicallysignificantrangefrom10-7to10-2M.
BAPTAAMesterisamembranepermeableformofBAPTAthatcanbeloadedintocellsbyincubation.BecauseoftherelativelylowwatersolubilityoftheAMester,themilddetergentPluronicF-127(catalogno.59004)isoftenusedasadispersingagenttofacilitateloADIng.BAPTAAMesteritselfdoesnotbindcalcium,butonceinsidethecellisconvertedintoBAPTAbycytoplasmicesterases.Seecatalogno.50001forinformationonBAPTA.
- ColorlesssolidsolubleinDMSO
- Storedesiccatedat-20°C
- C34H40N2O18
- MW:765
References
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