生物素/5X萤火虫荧光素酶裂解缓冲液/99923/15毫升
商品编号：
99923
品牌：
Biotium Inc
市场价：
¥380.00
美元价：
228.00
产品分类：
荧光染料
公司分类：
Fluorescent_dyes
联系Q Q：
3392242852
电话号码：
4000-520-616
电子邮箱：
info@ebiomall.com
商品介绍
5XFireflyLysisBufferisaconcentratedcelllysisbufferthatisdesignedforusewithBiotium’sFireflyLuciferaseAssayKit andFireflyLuciferaseAssayKit(Lyophilized).
5XFireflyLysisBufferisaconcentratedcelllysisbufferthatisdesignedforusewithBiotium’sFireflyLuciferaseAssayKit2.0(catalogno.30085)andFireflyLuciferaseAssayKit(Lyophilized)(catalogno.30075).Thesekitsaresuppliedwithsufficient5XFireflyLysisBuffertoperformthestatednumberofassayswithcellsgrownin96–24wellplates.5XFireflyLysisBufferissoldseparatelyforusewiththekitswhenmorelysisbuffer(e.g.>100uL/well)isrequired.
Note:5XFireflyLysisBufferisnotcompatIBLewithRenillaLuciferase.IfyourequireadditionallysisbufferforusewithBiotium’sRenillaLuciferaseAssayKit2.0(catalogno.30004)orFireflyandRenillaLuciferaseSingleTubeAssayKit(catalogno.30081),order5XPassiveLysisBuffer(catalogno.99912).
MoreLuciferaseKits:
| Catalognumber | Product | Description |
|---|---|---|
| 30085 | FireflyLuciferaseAssayKit2.0 | Flash-typefireflyluciferaseassaykit. |
| 30075 | FireflyLuciferaseAssayKit(Lyophilized) | Fireflyluciferaseassaykitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 99923 | 5XFireflyLysisBuffer | ExtralysisbufferforusewithFireflyLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30028 | Steady-Luc™FireflyHTSAssayKit | Glow-typefireflyluciferaseassaywithsignalhalf-lifeofabout3hoursforhigh-throughputscreeninginmulti-wellplates. |
| 30028L | Steady-LucFireflyHTSAssayKit(Lyophilized) | Steady-Luckitwithlyophilizedassaybufferforeconomicalroomtemperatureshippingandconvenientstorageat-20°C. |
| 30082 | RenillaLuciferaseAssayKit2.0 | Flash-typeRenillaluciferaseassaykit. |
| 99912 | 5XPassiveLysisBuffer | ExtralysisbufferforusewithRenillaLuciferaseAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30081 | FireflyandRenillaLuciferaseSingleTubeAssayKit | AssaykitformeasuringfireflyandRenillaactivitiessequentiallyinthesamesampleinasingletube. |
| 99821-100mL | 1XPassiveLysisBuffer2.0 | ExtralysisbufferforusewithFirefly&RenillaSingleTubeAssayKits,onlyrequiredifusingwellsizeslargerthan24-well. |
| 30020 | ATP-Glo™BioluminometricCellViABIlityAssayKit | Flash-typeluminescence-basedassayformeasuringcellviabilityorATPconcentration. |
| 22003 | MiniCellScrapers | Mini-cellscrapers,usefulforlysingcellsin96-well,48-well,or24-wellplates. |
References
- PLoSONE (2011),doi:10.1371/journal.pone.0025858
- BritishJPharmacol.(2014),doi:10.1111/bph.13014
- JNutritionalBiochem.(2013),http://www.jnutbio.com/article/S0955-2863(13)00143-5/abstract
- JBiol.Chem.291,110(2015).
品牌介绍
★EB（溴化乙锭）：溴化乙锭是一种高度灵敏的荧光染色剂，用于观察琼脂糖和聚丙烯酰胺凝胶中的DNA。溴化乙锭用标准302nm 紫外光透射仪激发并放射出橙红色信号，可用Polaroid 底片或带CCD 成像头的凝胶成像处理系统拍摄。 ★SYBR Green I/II是一种结合于所有dsDNA双螺旋小沟区域的具有绿色激发波长的染料。在游离状态下，SYBR Green I发出微弱的荧光，但一旦与双链DNA结合后，荧光大大增强。因此，SYBR Green I的荧光信号强度与双链DNA的数量相关，可以根据荧光信号检测出PCR体系存在的双链DNA数量。SYBR Green I 的最大吸收波长约为497nm，发射波长最大约为520nm。
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