Mix-n-StainFluorescentProtein&TandemDyeAntibodyLabelingKitsutilizearevolutionaryantibodylabelingtechnologythatallowsyoutoeasilylabelyourantibodywithAPC,R-PE,PerCPorAPC-CF750Tinabout4hours.Thelabelingproceduretoleratesmanycommonbuffercomponentsandantibodystabilizers(seetheproductprotocolunderdownloadsfordetails).Choosefromkitsizestolabel25-50ug,or50-100ugofyourantibody.Formoreinformation,pleasedownloadtheproductinformationsheetforthisproduct.
APCandR-PEbelongtothefamilyofphycobiliproteins,whicharederivedfromcyanobacteriaandeukaryoticalgae.
APChasabsorptionmaximaat650andemissionat660nm.Becauseofthehighfluorescencequantumyield(~0.68),APCisbyfarthebrightestdyeforthe650nmwavelengthregion.
R-PEhasabsorptionmaximaat496,546,and565nmrespectivelyandemissionat578nm.Becauseofthehighextinctioncoefficient(~1,960,000)andhighfluorescencequantumyield(~0.82),R-PEisbyfarthebrightestdyeforthe565nmwavelengthregion.
PerCPisasmall35kDAphycobiliproteinderivedfromredalgae. PerCPhasabsorptionmaximaat482nmandemissionat677nm.
TheMix-n-StainAPC-CF750TAntibodyLabelingKitisforlabelinganantibodywithtandemAPC-CF750T(Ex/Em650/780nm)formulti-colorflowcytometry.ItisdesignedfordetectionintheAPC-Cy®7channel.
ProductInformation
| Productdescription | Excitation/emission | Catalognumber | Reactionsize | Productprotocol |
|---|---|---|---|---|
| Mix-n-Stain™R-PEAntibodyLabelingKit | 496,546,565/ 578nm | 92298 | 25-50ugantibody | PI-Mix-n-StainR-PE |
| 92299 | 50-100ugantibody | |||
| Mix-n-Stain™APCAntibodyLabelingKit | 650/660nm | 92306 | 25-50ugantibody | PI-Mix-n-StainAPC |
| 92307 | 50-100ugantibody | |||
| Mix-n-Stain™PerCPAntibodyLabelingKit | 482/677nm | 92308 | 25-50ugantibody | PI-Mix-n-StainPerCP |
| 92309 | 50-100ugantibody | |||
| Mix-n-Stain™APC-CF™750AntibodyLabelingKit | 650/780nm(APC-Cy®7channel) | 92310 | 25-50ugantibody | PI-Mix-n-StainAPC-CF750 |
| 92311 | 50-100ugantibody |
References
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